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Year : 1995  |  Volume : 39  |  Issue : 1  |  Page : 8-11

Rapid Dot enzyme immuno assay for the quantitation of rabies antibodies.


Madras Veterinary College

Correspondence Address:
R Jayakumar
Madras Veterinary College

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Source of Support: None, Conflict of Interest: None


PMID: 8690480

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A rapid dot enzyme immuno assay (Dot ELISA) for rabies antibody estimation has been standardized in which nitrocellulose sheet has been used as a solid support absorbing a commercial tissue culture rabies vaccine as antigen. The test was compared with a standard plate ELISA test. The results were comparable and the student "t' test for proportion revealed that plate ELISA test is significantly better (P 0.05) when compared to Dot ELISA for the number of sera with titre < 0.5 Iu/ml and in the case of > 0.5 IU/ml Dot ELISA is found to be better over plate ELISA test at the same significance level.


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